Journal: bioRxiv

Article Title: Phase separation and molecular ordering of the prion-like domain of the thermosensory protein EARLY FLOWERING 3

doi: 10.1101/2023.03.12.532276

Figure Lengend Snippet: Coupled AFM and confocal fluorescence microscopy of ELF3 PrLD -GFP constructs. AFM topography mapping is shown at left, fluorescence of the same sample is shown at middle and stiffness measurements are shown at right. Alignment between the AFM and confocal micro-scope was within 1-2 microns for the two microscopes. Scale bar = 5 μm.

Article Snippet: AFM coupled wide-field fluorescence microscope was developed in-house, allowing AFM imaging and epifluorescence or Total Internal Reflection Fluorescence (TIRF) imaging (Supplementary Fig. S3b) , . and based on a Nanowizard 4 (JPK Instruments, Bruker) mounted on a Zeiss inverted optical microscope.

Techniques: Fluorescence, Microscopy, Construct

Journal: bioRxiv

Article Title: Phase separation and molecular ordering of the prion-like domain of the thermosensory protein EARLY FLOWERING 3

doi: 10.1101/2023.03.12.532276

Figure Lengend Snippet: Wide-field total internal reflection fluorescence microscopy and high-resolution AFM of ELF3-GFP PrLD gel droplets. A) Young’s modulus measurements of the droplets showing in-homogeneous stiffness within individual droplets, scale bar = 1 μm. B) Fluorescence images of ELF3 PrLD Q0 (left), Q7 (middle) and Q20 (right), scalebar = 1 μm. Areas of interest are boxed and shown in close-up in (c). C) AFM topography of the droplets in a, scalebar = 1 μm. D) Height profile measurements across individual droplets (blue lines in c) showing a step pattern between flat layers.

Article Snippet: AFM coupled wide-field fluorescence microscope was developed in-house, allowing AFM imaging and epifluorescence or Total Internal Reflection Fluorescence (TIRF) imaging (Supplementary Fig. S3b) , . and based on a Nanowizard 4 (JPK Instruments, Bruker) mounted on a Zeiss inverted optical microscope.

Techniques: Fluorescence, Microscopy